Short Note: Isolation and Characterization of 12 Polymorphic Microsatellite Markers in Engelhardia roxburghiana (Juglandaceae)
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چکیده
منابع مشابه
Isolation and characterization of 20 polymorphic microsatellite markers for Juglans mandshurica (Juglandaceae)1
UNLABELLED PREMISE OF THE STUDY Fifty microsatellite loci were developed for the endangered species Juglans mandshurica to investigate its genetic diversity and population structure. • METHODS AND RESULTS In all, 50 microsatellite markers were isolated from J. mandshurica, using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol. Twenty of these polymorphic marker...
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Twelve polymorphic microsatellite loci were isolated from an (AC)n- and (AG)n-enriched DNA library for the endemic Chinese frog Pelophylax hubeiensis (Ranidae). The number of alleles per locus ranged from two to eight, with a mean of 5.17. The observed and expected heterozygosities ranged from 0.226 to 0.839 and from 0.204 to 0.826, with means of 0.568 and 0.656, respectively. No significant li...
متن کاملIsolation and characterization of microsatellite markers in Beilschmiedia roxburghiana (Lauraceae)1
UNLABELLED PREMISE OF THE STUDY Although there are as many as 250 species in the genus Beilschmiedia, their genetic diversity has been poorly investigated. Our objective was to develop microsatellite markers for B. roxburghiana to study its genetic diversity for the sustainable management of this species. • METHODS AND RESULTS Using the microsatellite-enriched library and PCR-based screeni...
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Microsatellite enriched library of Hyriopsis cumingii was constructed according to the strong affinity between biotin and streptavidin. One hundred clones with foreign inserts were sequenced and 65 clones were screened for usable microsatellites, of which 36 were deemed unique, of sufficient length (more than 8 repeats) and possessed adequate flanking regions for primer designment. Among 36 pri...
متن کاملIsolation and characterization of 13 new microsatellite markers in the triangle mussel (Hyriopsis cumingii)
Microsatellite enriched library of Hyriopsis cumingii was constructed according to the strong affinity between biotin and streptavidin. One hundred clones with foreign inserts were sequenced and 65 clones were screened for usable microsatellites, of which 36 were deemed unique, of sufficient length (more than 8 repeats) and possessed adequate flanking regions for primer designment. Among 36 pri...
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ژورنال
عنوان ژورنال: Silvae Genetica
سال: 2014
ISSN: 2509-8934
DOI: 10.1515/sg-2014-0014